Journal
ACCOUNTS OF CHEMICAL RESEARCH
Volume 38, Issue 7, Pages 574-582Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ar040136s
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We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within similar to 1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.
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