Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 24, Issue 3, Pages 249-253Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2004.12.004
Keywords
decolorization; yeast; manganese-dependent peroxidase; azo dye; C.I Reactive Black 5
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The optimum conditions for decolorization of an azo dye, C.I. Reactive Black 5 (RB5) and the kinetic characteristics of manganese-dependent peroxidase (MnP) production by yeast isolate, Debaryomyces polymorphus, were investigated. D. polymorphus could completely degrade 200 mg l(-1) of non-hydrolyzed and hydrolyzed C.I. Reactive Black 5 within 24 h of cultivation at an inoculum size of 1.4 g l(-1) wet cells in 50 ml medium consisting of 5 g l(-1) glucose and 0.5-1.0 g l(-1) ammonium sulphate (pH 5-7). In addition, the MnP activities during the cultivation were evaluated in the absence and presence of 200 mg l(-1) C.I. Reactive Black 5. Maximum activity of MnP (1555.6 U l(-1)) was detected at 24 h cultivation in the presence of the dye, and a significant reduction of the enzyme activity was observed thereafter. The presence of C.I. Reactive Black 5 in the culture was found to be indispensable to the production of MnP by D. polymorphus. A good correlation was found between the dye degradation and the enzyme production. (C) 2005 Elsevier B.V. All rights reserved.
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