4.7 Article

A microtubule-dependent zone of active RhoA during cleavage plane specification

Journal

JOURNAL OF CELL BIOLOGY
Volume 170, Issue 1, Pages 91-101

Publisher

ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200501131

Keywords

-

Categories

Funding

  1. NIGMS NIH HHS [R01 GM052932, GM52932, GM66050, P50 GM066050] Funding Source: Medline

Ask authors/readers for more resources

Cytokinesis in animal cells results from the assembly and constriction of a circumferential array of actin filaments and myosin-2. Microtubules of the mitotic apparatus determine the position at which the cytokinetic actomyosin array forms, but the molecular mechanisms by which they do so remain unknown. The small GTPase RhoA has previously been implicated in cytokinesis. Using four-dimensional microscopy and a probe for active RhoA, we show that active RhoA concentrates in a C precisely bounded zone before cytokinesis and is independent of actin assembly. Cytokinetic RhoA activity zones are common to four echinoderm species, the vertebrate Xenopus laevis, and the highly asymmetric cytokinesis accompanying meiosis. Microtubules direct the formation and placement of the RhoA activity zone, and the zone is repositioned after physical spindle displacement. We conclude that microtubules specify the cytokinetic apparatus via a dynamic zone of local RhoA activity.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available