Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 280, Issue 27, Pages 25907-25912Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M412476200
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- NIGMS NIH HHS [R01 GM26494] Funding Source: Medline
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The current model for cell- to- cell movement of plant viruses holds that transport requires virus- encoded movement proteins that intimately associate with endoplasmic reticulum membranes. We have examined the early stages of the integration into endoplasmic reticulum membranes of a double- spanning viral movement protein using photocross- linking. We have discovered that this process is cotranslational and proceeds in a signal recognition particle- dependent manner. In addition, nascent chain photocross- linking to Sec61 alpha and translocating chain- associated membrane protein reveal that viral membrane protein insertion takes place via the translocon, as with most eukaryotic membrane proteins, but that the two transmembrane segments of the viral protein leave the translocon and enter the lipid bilayer together.
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