Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 517, Issue 3, Pages 246-251Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2005.05.011
Keywords
nicotinic receptor; lymphocyte; immune response
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The presence of nicotinic acetylcholine receptors (nicotinic receptors) composed of either alpha 7 or alpha 4 and beta 2 subunits is revealed in B lymphocytes by means of radioligand binding assay and Cell ELISA. Mouse B lymphocytes contained 12,200 3200 of epibatidine-binding sites and 3130 750 of alpha-Bungarotoxin-binding sites per cell. Mice lacking nicotinic receptor subunits alpha 4, beta 2 or alpha 7 had less serum IgG and IgG-producing cells in the spleen, but showed stronger immune response to both protein antigen in vivo and CD40-specific antibody in vitro than wild-type mice. Anti-CD40-stimulated proliferation of B lymphocytes from beta 2 knockout, but not wild-type mice was inhibited with nicotine. Our results indicate that signalling through nicotinic receptors affects both the pre-immune state and activation of B lymphocytes in the immune response, possibly via CD40-dependent pathway. This could contribute to immune depression found in tobacco smokers. (c) 2005 Elsevier B.V. All rights reserved.
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