Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 28, Pages 9796-9801Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0502917102
Keywords
binding coefficient; multiple sites; transcription factors
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Funding
- NHGRI NIH HHS [HG 01506, R01 HG001506] Funding Source: Medline
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We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage A DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to be 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.
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