Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 81, Issue 2, Pages 235-243Publisher
WILEY
DOI: 10.1002/jnr.20560
Keywords
energy dependence; proton dependence; iron dependence; sodium dependence; astrocyte-conditioned medium
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Funding
- NIEHS NIH HHS [ES10563] Funding Source: Medline
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Manganese (Mn), an essential nutrient, is neurotoxic at high levels and has been associated with the development of a parkinsonian syndrome termed manganism. Currently, the mechanisms responsible for transporting Mn across the blood-brain barrier (BBB) are unknown. By using rat brain endothelial 4 (RBE4) cell monolayers cultured in astrocyte-conditioned media (ACM), we examine the effects of temperature, energy, proton (pH), iron (Fe), and sodium (Na+) dependence on Mn transport. Our results suggest that Mn transport is temperature, energy, and pH dependent, but not Fe or Na+-dependent. These data suggest that Mn transport across the BBB is an active process, but they also demonstrate that the presence of ACM in endothelial cell cultures decreases the permeability of these cells to Mn, reinforcing the use of ACM or astrocyte cocultures in studies examining metal transport across the BBB. (c) 2005 Wiley-Liss, Inc.
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