Journal
REGULATORY PEPTIDES
Volume 129, Issue 1-3, Pages 119-124Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.regpep.2005.02.003
Keywords
NPY; mitogen-activated protein kinase; cell proliferation; peptide YY; pancreatic polypeptide; SK-N-MC
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Funding
- PHS HHS [40498] Funding Source: Medline
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Expression of neuropeptide Y (NPY) receptors in human SK-N-MC neuroblastoma cells was investigated. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that Y1, Y4, and Y5 receptors were expressed in these cells. Expression was confirmed by Western blot and immunocytochemistry demonstrated abundant presence of all three receptors on cell membranes. NPY peptide was also expressed in these cells, but other members of the larger peptide family (peptide YY and pancreatic polypeptide) were not expressed. Incubation with U0126, a specific mitogen-activated protein kinase (MAPK) inhibitor, decreased cell number in serum-free medium culture. Since NPY activates MAPK via different subtypes of NPY receptors, results suggest that endogenously expressed NPY may control proliferation of these cells through a paracrine/autocrine mechanism. (c) 2005 Elsevier B.V. All rights reserved.
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