Journal
BIOSENSORS & BIOELECTRONICS
Volume 21, Issue 1, Pages 217-222Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.10.008
Keywords
electron transfer; cytochrome; gold nanoparticles; cholesterol; biosensors; screen-printed
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This paper is concerned with an investigation of electron transfer between cytochrome P450scc (CYP11A1) and gold nanoparticles immobilised on rhodium-graphite electrodes. Thin films of gold nanoparticles were deposited onto the rhodium-graphite electrodes by drop casting. Cytochrome P450scc was deposited onto both gold nanoparticle modified and bare rhodium-graphite electrodes. Cyclic voltammetry indicated enhanced activity of the enzyme at the gold naroparticle modified surface. The role of the nanoparticles in mediating electron transfer to the cytochrome P450scc was verified using ac impedance spectroscopy. Equivalent circuit analysis of the impedance spectra was performed and the values of the individual components estimated. On addition of aliquots of cholesterol to the electrolyte bioelectrocatalytic reduction currents were obtained. The sensitivity of the nanoparticle modified biosensor to cholesterol was 0. 13 mu A mu M-1 in a detection range between 10 and 70 mu M of cholesterol. This confirms that gold nanoparticles enhance electron transfer to the P450scc when present on the rhodium-graphite electrodes. (c) 2004 Elsevier B.V. All rights reserved.
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