Journal
FEMS MICROBIOLOGY LETTERS
Volume 248, Issue 2, Pages 207-215Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.05.032
Keywords
Bifidobacterium longum; stress; 2D-PAGE; [S-35] labelling; MALDI-TOF/TOF
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Experimental conditions for efficient protein radiolabelling and two-dimensional gel electrophoresis were developed for Bifidobacterium longum. Using these tools, protein synthesis in cells before and after heat-shock and bile salts treatment was investigated. Following heat-stress, 13 proteins were upregulated, of which HtrA, DnaK and GroEL were also moderately induced by bile salts, indicating close relationship between the heat and bile salts responses in bifidobacteria. Our work indicated that, as a consequence of prolonged heat-stress, HtrA undergoes sequential modification and proteolysis, and that this mechanism could be employed by bifidobacteria to respond to heat-stress. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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