Journal
MACROMOLECULAR RAPID COMMUNICATIONS
Volume 26, Issue 14, Pages 1168-1172Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/marc.200500141
Keywords
bienzyme system; melamine formaldehyde; microencapsulation; microspheres; polyelectrolytes
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Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed by the partial decomposition of the core. Peroxidase and glucose oxidase were immobilized in the prepared microspheres. Retention of enzymatic activity of the peroxidase/glucose oxidase system incorporated into the microspheres was demonstrated, Applicability of the bienzyme system immobilized in the microspheres for kinetic glucose assay was shown.
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