Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 80, Issue 21, Pages 6843-6852Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02465-14
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Funding
- Australian Research Council (ARC) [DP120103957]
- National Health and Medical Research Council (NHMRC) [1022240]
- Channel 7 Children's Research Foundation [13661]
- Australian Cystic Fibrosis Research Trust scholarship
- [565526]
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In microaerophilic or anaerobic environments, Pseudomonas aeruginosa utilizes nitrate reduction for energy production, a process dependent on the availability of the oxyanionic form of molybdenum, molybdate (MoO42-). Here, we show that molybdate acquisition in P. aeruginosa occurs via a high-affinity ATP-binding cassette permease (ModABC). ModA is a cluster D-III solute binding protein capable of interacting with molybdate or tungstate oxyanions. Deletion of the modA gene reduces cellular molybdate concentrations and results in inhibition of anaerobic growth and nitrate reduction. Further, we show that conditions that permit nitrate reduction also cause inhibition of biofilm formation and an alteration in fatty acid composition of P. aeruginosa. Collectively, these data highlight the importance of molybdate for anaerobic growth of P. aeruginosa and reveal novel consequences of nitrate reduction on biofilm formation and cell membrane composition.
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