Journal
OPTICS EXPRESS
Volume 13, Issue 15, Pages 5883-5889Publisher
OPTICAL SOC AMER
DOI: 10.1364/OPEX.13.005883
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We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either alpha-streptavidin or alpha-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes. (C) 2005 Optical Society of America.
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