Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 127, Issue 29, Pages 10314-10322Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja0520969
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We performed polarization-resolved surface second harmonic generation (SHG) experiments on thin films of Collagen I and IV molecules, as well as conventional CID measurements. We found that Collagen IV presents little CD and no SHG optical activity, whereas Collagen I exhibits large chiroptical effects involving both one-electron and excitonic coupling mechanisms. We estimated that these chiral components enhance the SHG signal from fibrillar Collagen in biological tissues by typically a factor of 2. By comparing the distinct behaviors of collagens I and IV in SHG microscopy and in surface SHG experiments, we concluded that SHG microscopy is a sensitive probe of the micrometer-scale structural organization of Collagen in biological tissues.
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