Journal
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
Volume 64, Issue 1, Pages 38-45Publisher
ELSEVIER
DOI: 10.1016/j.jbbm.2005.05.003
Keywords
cadmium sulfide nanoparticles; direct electrochemistry; hemoglobin
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Hemoglobin (Hb) is immobilized with cadmium sulfide (US) nanoparticles (NPs) on pyrolytic graphite (PG) electrode to characterize the electrochemical reactivity and peroxidase activity of the protein. The result demonstrates that fine redox waves of Hb can be achieved after this protein is entrapped in CdS NPs. Meanwhile, the protein can exhibit nice catalytic activity towards hydrogen peroxide (H2O2). Linear relationship between the reductive peak current and the H2O2 concentration has been obtained from 5.0 x 10(-6) to 4.0 x 10(-4) mol/L, on the basis of which a new kind of H2O2 biosensor might be developed in the future. (c) 2005 Elsevier B.V. All rights reserved.
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