4.6 Article

Disruption of Putrescine Biosynthesis in Shewanella oneidensis Enhances Biofilm Cohesiveness and Performance in Cr(VI) Immobilization

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 80, Issue 4, Pages 1498-1506

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.03461-13

Keywords

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Funding

  1. National Research Foundation under its Research Centre of Excellence Programme
  2. Ministry of Education Singapore under Research Centre of Excellence Programme
  3. Singapore Centre on Environmental Life Sciences Engineering (SCELSE) [M4330005.C70]
  4. College of Engineering, Nanyang Technological University, Singapore [M4080847.030]

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Although biofilm-based bioprocesses have been increasingly used in various applications, the long-term robust and efficient biofilm performance remains one of the main bottlenecks. In this study, we demonstrated that biofilm cohesiveness and performance of Shewanella oneidensis can be enhanced through disrupting putrescine biosynthesis. Through random transposon mutagenesis library screening, one hyperadherent mutant strain, CP2-1-S1, exhibiting an enhanced capability in biofilm formation, was obtained. Comparative analysis of the performance of biofilms formed by S. oneidensis MR-1 wild type (WT) and CP2-1-S1 in removing dichromate (Cr2O72-), i.e., Cr(VI), from the aqueous phase showed that, compared with the WT biofilms, CP2-1-S1 biofilms displayed a substantially lower rate of cell detachment upon exposure to Cr(VI), suggesting a higher cohesiveness of the mutant biofilms. In addition, the amount of Cr(III) immobilized by CP2-1-S1 biofilms was much larger, indicating an enhanced performance in Cr(VI) bioremediation. We further showed that speF, a putrescine biosynthesis gene, was disrupted in CP2-1-S1 and that the biofilm phenotypes could be restored by both genetic and chemical complementations. Our results also demonstrated an important role of putrescine in mediating matrix disassembly in S. oneidensis biofilms.

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