Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 79, Issue 11, Pages 3516-3521Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00409-13
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Funding
- Next-Generation BioGreen 21 Program (SSAC) of the Rural Development Administration [PJ00948302]
- Converging Research Center Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology, Republic of Korea [20090082333]
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A UDP-glycosyltransferase from Bacillus licheniformis was exploited for the glycosylation of phloretin. The in vitro glycosylation reaction confirmed the production of five phloretin glucosides, including three novel glucosides. Consequently, we demonstrated the application of the same glycosyltransferase for the efficient whole-cell biocatalysis of phloretin in engineered Escherichia coli.
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