Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 79, Issue 19, Pages 6176-6179Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02015-13
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- Max Planck Society
- Fonds der Chemischen Industrie
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Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.
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