Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 79, Issue 14, Pages 4503-4508Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00866-13
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Funding
- China Scholarship Council [2010605032]
- NWO-ESF EuroSynbio program
- SNN (Province of Groningen) [T2006]
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Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and P-czcD. P-czcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.
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