4.6 Article

Increased Furan Tolerance in Escherichia coli Due to a Cryptic ucpA Gene

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 7, Pages 2452-2455

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.07783-11

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Funding

  1. Myriant Corp.
  2. Department of Energy [DE-FG3608GO88142]
  3. Department of Agriculture [2011-10006-30358]

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Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.

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