Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 7, Pages 2452-2455Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.07783-11
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Funding
- Myriant Corp.
- Department of Energy [DE-FG3608GO88142]
- Department of Agriculture [2011-10006-30358]
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Expression arrays were used to identify 4 putative oxidoreductases that were upregulated (>3-fold) by furfural (15 mM, 15 min). Plasmid expression of one (ucpA) increased furan tolerance in ethanologenic strain LY180 and wild-type strain W. Deleting ucpA decreased furfural tolerance. Although the mechanism remains unknown, the cryptic ucpA gene is now associated with a phenotype: furan resistance.
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