Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 8, Pages 3010-3013Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.07501-11
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Funding
- Flemish Fund for Scientific Research (FWO) [V4.321.10N]
- Dutch Organization for Scientific Research (NWO) [040.11.208]
- Geconcerteerde Onderzoeksactie (GOA) of Ghent University [BOF09/GOA/005]
- ERC [232937]
- KRW [09035]
- Netherlands Organization for Scientific Research [863.11.003]
- Darwin Center for Biogeology
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A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80 degrees C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of Candidatus Kuenenia stuttgartiensis.
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