4.6 Article

Multiplex PCR Assay Targeting a Diguanylate Cyclase-Encoding Gene, cgcA, To Differentiate Species within the Genus Cronobacter

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 79, Issue 2, Pages 734-737

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02898-12

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Funding

  1. National Association for Equal Opportunity in Higher Education internship program fellowship, Washington, DC
  2. oint Institute of Food Safety and Applied Nutrition student internship program, University of Maryland, College Park, MD

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In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

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