4.6 Article

Clostridium carboxidivorans Strain P7T Recombinant Formate Dehydrogenase Catalyzes Reduction of CO2 to Formate

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 79, Issue 2, Pages 741-744

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02886-12

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Funding

  1. CSIRO Flagship Collaboration Fund
  2. ARC Centre of Excellence for Free Radical Chemistry and Biotechnology

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Recombinant formate dehydrogenase from the acetogen Clostridium carboxidivorans strain P7(T), expressed in Escherichia coli, shows particular activity towards NADH-dependent carbon dioxide reduction to formate due to the relative binding affinities of the substrates and products. The enzyme retains activity over 2 days at 4 degrees C under oxic conditions.

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