Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 11, Pages 4051-4056Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.07994-11
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- Norwegian-Canadian Holos project
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The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification.
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