4.6 Article

High-Throughput PCR Assays To Monitor Wolbachia Infection in the Dengue Mosquito (Aedes aegypti) and Drosophila simulans

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 13, Pages 4740-4743

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00069-12

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Funding

  1. Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative
  2. CSIRO Cluster Collaboration Fund Urbanism, Climate Change and Health
  3. fellowships from the Australian Research Council

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We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.

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