Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 15, Pages 5440-5443Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00844-12
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Funding
- KORDI [PE98802]
- Marine and Extreme Genome Research Center
- Ministry of Land, Transport, and Maritime Affairs, Korea
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20090213] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
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