Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 18, Pages 6722-6725Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.05441-11
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- NDRI
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This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity in the LSU rDNA for species-level differentiation.
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