Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 8, Pages 2727-2733Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02454-10
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- BioEnergy Science Center (BESC) at Oak Ridge National Laboratory, a Department of Energy Bioenergy Research Center
- UCLA-DOE Institute for Genomics and Proteomics
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Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis. Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism.
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