Journal
JOURNAL OF SEPARATION SCIENCE
Volume 28, Issue 13, Pages 1502-1511Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200400044
Keywords
HPLC; stationary phase; modified nucleosides; selectivity; separation mechanism
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Analysis of the modified nucleosides is particularly important in the medical area because of a possibility of cancerogenic processes studies. The aim of this work was to study the selectivity tuning of modified nucleosides. through the investigations of interactions analyte (modified nucleoside) double left right arrow stationary phase double left right arrow mobile phase. A series of homemade stationary phases with different surface properties has been utilized. All of them contain various interaction sites such as: cholesterol (SG-CHOL); n-acylamide (SG-CHOL, SG-AP); aminopropyl (SG-CHOL, SG-AP, SG-NH2, SG-MIX); cyanopropyl, phenyl, octyl (SG-MIX), octadecyl (SG-MIX, SG-C-18) and silanols localized on the silica gel surface of all packings. The attempt to predict the main interactions responsible for the retention between nucleosides. and stationary phase ligands was done on the basis of the elemental analysis, and proportional part of an individual ligand bonded to silica surface results, In order to study the influence of different packing types on the analyzed nucleosides retention, the relationship between pH of the mobile phase buffer and the selectivity of a stationary phase was investigated.
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