Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 18, Pages 6703-6705Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.05712-11
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Funding
- National Institutes of Health [2R15HL079992-02]
- University of Texas of the Permian Basin
- University of Texas System Louis Stokes Alliance
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We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
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