4.6 Article

Novel Multiplex Single Nucleotide Polymorphism-Based Method for Identifying Epidemic Clones of Listeria monocytogenes

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 17, Pages 6290-6294

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00429-11

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Funding

  1. MIUR-Ministero dell'Istruzione, dell'Universita e della Ricerca
  2. Regione Piemonte

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A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.

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