4.6 Article

Development of a 16S rRNA Gene Primer and PCR-Restriction Fragment Length Polymorphism Method for Rapid Detection of Members of the Genus Megasphaera and Species-Level Identification

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 15, Pages 5533-5535

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00359-11

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The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism ( RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.

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