Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 16, Pages 5604-5609Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02974-10
Keywords
-
Categories
Funding
- INRA
- Regional Council Provence Alpes Cote d'Azur
- Agence Nationale de la Recherche (ANR) (France) [ANR-05-PNRA-013]
Ask authors/readers for more resources
In this study, growth rates and lag times of the five RNA helicase-deleted mutants of Bacillus cereus ATCC 14579 were compared to those of the wild-type strain under thermal, oxidative, and pH stresses. Deletion of cshD and cshE had no impact under any of the tested conditions. Deletion of cshA, cshB, and cshC abolished growth at 12 degrees C, confirming previous results. In addition, we found that each RNA helicase had a role in a specific temperature range: deletion of cshA reduced growth at all the tested temperatures up to 45 degrees C, deletion of cshB had impact below 30 degrees C and over 37 degrees C, and deletion of cshC led mainly to a cold-sensitive phenotype. Under oxidative conditions, deletion of cshB and cshC reduced growth rate and increased lag time, while deletion of cshA increased lag time only with H(2)O(2) and reduced growth rate at a high diamide concentration. Growth of the Delta cshA strain was affected at a basic pH independently of the temperature, while these conditions had a limited effect on Delta cshB and Delta cshC strain growth. The RNA helicases CshA, CshB, and CshC could participate in a general adaptation pathway to stressful conditions, with a stronger impact at low temperature and a wider role of CshA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available