Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 1, Pages 185-193Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.06192-11
Keywords
-
Categories
Funding
- Fund for Scientific Research (FWO) of Flanders, Belgium
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen)
- Fund for Scientific Research, Flanders (FWO) [G.0236.07]
- TEKES (Finnish Funding Agency for Technology and Innovation) [201/08]
- Academy of Finland [118165]
- Valio Ltd., Research and Development, Helsinki, Finland
Ask authors/readers for more resources
Lactobacillus rhamnosus GG, a probiotic with good survival capacity in the human gut, has well-documented adhesion properties and health effects. Recently, spaCBA-encoded pili that bind to human intestinal mucus were identified on its cell surface. Here, we report on the phenotypic analysis of a spaCBA pilus knockout mutant in comparison with the wild type and other adhesin mutants. The SpaCBA pilus of L. rhamnosus GG showed to be key for efficient adherence to the Caco-2 intestinal epithelial cell (IEC) line and biofilm formation. Moreover, the spaCBA mutant induces an elevated level of interleukin-8 (IL-8) mRNA in Caco-2 cells compared to the wild type, possibly involving an interaction of lipoteichoic acid with Toll-like receptor 2. In contrast, an L. rhamnosus GG mutant without exopolysaccharides but with an increased exposure of pili leads to the reduced expression of IL-8. Using Transwells to partition bacteria from Caco-2 cells, IL-8 induction is blocked completely regardless of whether wild-type or mutant L. rhamnosus GG cells are used. Taken together, our data suggest that L. rhamnosus GG SpaCBA pili, while promoting strong adhesive interactions with IECs, have a functional role in balancing IL-8 mRNA expression induced by surface molecules such as lipoteichoic acid.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available