4.6 Article

pmoA Primers for Detection of Anaerobic Methanotrophs

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 11, Pages 3877-3880

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02960-10

Keywords

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Funding

  1. Foundation for Applied Research [07736]
  2. Chinese Academy of Science-Royal Netherlands Academy of Arts and Sciences
  3. ERC [2322937]
  4. Darwin Center for Biogeology
  5. Spanish Ministry of Science and Innovation
  6. NSF [EAR-1024900]
  7. USGS
  8. Division Of Earth Sciences
  9. Directorate For Geosciences [1329284, 1024900] Funding Source: National Science Foundation
  10. Division Of Earth Sciences
  11. Directorate For Geosciences [1024662] Funding Source: National Science Foundation

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Published pmoA primers do not match the pmoA sequence of Candidatus Methylomirabilis oxyfera, a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of Ca. Methylomirabilis oxyfera-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.

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