Journal
JOURNAL OF PINEAL RESEARCH
Volume 39, Issue 1, Pages 27-33Publisher
WILEY
DOI: 10.1111/j.1600-079X.2005.00208.x
Keywords
antioxidant; conjugated diene; hydroperoxide; lipid peroxidation; melatonin; pinoline derivative
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This study aimed at investigating the in vitro protective effects of GWC22, a novel pinoline derivative [6-ethyl-1-(3-methoxyphenyl)-2-propyl-1,2,3,4- tetrahydro-beta-carboline] chlorhydrate, against radiation-induced oxidation of linoleate initiated by hydroxyl radicals((OH)-O-center dot). Using linoleate micelles (10(-2) m) as lipid model, two indexes of peroxidation have been measured, i.e. conjugated dienes and hydroperoxides. Similar determinations were performed with melatonin in order to compare the protective effects of the two compounds. It was observed that, the higher the concentration of GWC22 ( or melatonin) (3 x 10(-5) to 10(-4) m), the stronger the antioxidant ability. In these in vitro assays, GWC22 showed a better antioxidant effect than melatonin for a given antioxidant concentration. A reaction scheme has been proposed to explain the inhibitory effect of an antioxidant via the propagating steps of the lipid peroxidation. Indeed, we have suggested that melatonin and GWC22 may compete with the fatty acid to scavenge lipid peroxyl radicals (LOO center dot). We have estimated a lower limit for the LOO center dot rate constant for GWC22 (>= 1.4 x 10(5)/ m/s) and for melatonin (>= 2.8 x 10(4)/ m/ s) assuming that the k-value of the propagating step in linoleate (LOO center dot + linoleate) was 1.4 x 10(3)/ m/ s. The difference of reactivity between melatonin and GWC22 in this model system is assumed to be related to their relative lipophilicity.
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