Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 12, Pages 4223-4225Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02289-10
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- National Institute of General Medical Sciences [R43/R44GM076843]
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The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of < 5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH > 8.3 for flame orange.
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