4.6 Article

Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 71, Issue 8, Pages 4925-4929

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.71.8.4925-4929.2005

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Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.

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