Journal
NATURE BIOTECHNOLOGY
Volume 23, Issue 8, Pages 988-994Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nbt1120
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Funding
- NIGMS NIH HHS [1R01GM068110, R01 GM068110] Funding Source: Medline
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The DNA-binding specificities of transcription factors can be used to computationally predict cis-regulatory modules (CRMs) that regulate gene expression(1). However, the absence of specificity data for the majority of transcription factors limits the widespread implementation of this approach. We have developed a bacterial one-hybrid system that provides a simple and rapid method to determine the DNA-binding specificity of a transcription factor. Using this technology, we successfully determined the DNA-binding specificity of seven previously characterized transcription factors and one novel transcription factor, the Drosophila melanogaster factor Odd-skipped. Regulatory targets of Odd-skipped were successfully predicted using this information, demonstrating that the data produced by the bacterial one-hybrid system are relevant to in vivo function.
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