4.6 Article

Practical Method for Extraction of PCR-Quality DNA from Environmental Soil Samples

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 13, Pages 4571-4573

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02825-09

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Funding

  1. Association of Public Health Laboratories (APHL)
  2. CDC

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Methods for the extraction of PCR-quality DNA from environmental soil samples by using pairs of commercially available kits were evaluated. Coxiella burnetii DNA was detected in spiked soil samples at <1,000 genome equivalents per gram of soil and in 12 (16.4%) of 73 environmental soil samples.

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