Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 3, Pages 1118-1122Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01733-10
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Funding
- National Science Foundation [CBET-0606894]
- Office of Science, Department of Energy [DE-FG02-07ER64495]
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Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.
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