Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 14, Pages 4914-4917Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00958-10
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Funding
- DOE BioEnergy Science Center (BESC)
- USDA Bioprocessing and Biodesign Center
- DuPont Young Professor Award
- China Scholar Council
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A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant enzymes on soluble and solid cellulosic substrates, suggesting the high complexity of cellulase engineering. The best mutant had 92%, 36%, and 46% longer half-lives at 60 degrees C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively.
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