Journal
JOURNAL OF PARASITOLOGY
Volume 91, Issue 4, Pages 965-967Publisher
AMER SOC PARASITOLOGISTS
DOI: 10.1645/GE-3457RN.1
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Serum samples from 58 Pacific bottlenose dolphins (Tursiops aduncus) from the Solomon Islands were tested for the IgG antibody to Toxoplasma gondii by the latex agglutination test (LAT), enzyme-linked immunosorbent assay (ELISA), and immunoblotting. The ELISA cut-off value was taken as OD >= 0.276, and the final dilution ratio. recognized as positive, was represented by the end titer. In 25 of 58 samples. no antibody activity was detected by LAT and ELISA. In 8 of 58 samples. anti-T gondii IgG antibodies were detected by both LAT and ELISA. with titers of greater than 1 : 64 and 1 : 160, respectively. By immunoblotting, the 8 serum samples producing higher titers showed specific antibody IgG binding to several antigens on the T. gondii lane. but not on the Neospora caninum lane. No specific bands were noted on the lanes for either parasite in the 25 serum Samples for which no antibody activity seas detected. The specific binding of I,,G antibodies to T. gondii antigens observed for serum samples producing higher titers suggests that Pacific bottlenose dolphins front the Solomon islands are exposed to T. gondii.
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