Journal
JOURNAL OF MASS SPECTROMETRY
Volume 40, Issue 8, Pages 1000-1004Publisher
WILEY
DOI: 10.1002/jms.859
Keywords
salicylic acid; solid-phase extraction; desorption/ionization on silicon mass spectrometry; quantitative analysis
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The applicability of the matrix-free laser desorption/ionization on silicon mass spectrometry (DIOS-MS) to measuring serum drug levels was examined by analyzing serum salicylic acid. The optimized and simple solid-phase extraction (SPE) allowed good recovery, 88.9 +/- 5.8%, for 1.4 mM (200 mg/L) of salicylic acid in serum. The negative ion MS allowed measurements of deprotonated molecules without interference from other signals. Using a deuterium-labeled internal standard, good linearity was obtained in the 0.14 to 4.2 mM (20-600 mg/L) range, which was sufficient for monitoring the therapeutic anti-inflammatory dose. SPE followed by DIOS-MS is anticipated to be a method of measuring drug levels in blood and may allow high throughput analysis. Copyright (c) 2005 John Wiley & Sons, Ltd.
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