Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 17, Pages 6001-6005Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00770-10
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Funding
- Shanghai Municipal Committee of Agriculture [2008-7-5]
- Shanghai Basic Research Project [08JC1418000]
- 863 Programs [2006AA10Z117, 2006AA06Z358, 2008AA10Z401]
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Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Ochrobactrum anthropi was identified, cloned, and overexpressed, and the enzyme was purified to homogeneity. Furthermore, site-directed mutagenesis was employed to assess the role of single amino acid residues in glyphosate resistance.
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