Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 10, Pages 3398-3400Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02373-09
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Funding
- USDA Current Research Information System (CRIS) [5325-42000-044-00D]
- USDA Cooperative State Research, Education, and Extension Service (CSREES) [2006-01240, 2007-02029]
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Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA.
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