Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques

Objective: To test the hypothesis that couples with sperm chromatin structure assay (SCSA) DNA frragmentation index (DFI) and high DNA stainability (HDS) as measured by the SCSA, affect fertilization, cleavage, implantation, and pregnancy rates in IVF cycles. Design: Prospective clinincal study. Setting: Academic human reproductive laboratory. Patients(s): One hundred couples undergoing IVF with conventional insemination or intracytoplasmic sperm injection. Interventions: Testing with SCSA was performed by SCSA Diagnostics (Brookings, south Dakota) on a semen aliquot taken from used for ART. Main Outcome Measure(s): Relating total DFI and HDS to convential semen parameters and cylce specific outcomes after ART. Results: Nine of nineteen couples achieved clinical pregnancy when DFI was >= 27%, and 2 of 22 couples achieved clinical pregnancy when DFI was <= 9%. One of nine couples achieved clinical pregnancy with HDS > 17%. The DFI was negatively correlated with sperm density (r = -0.23, P <.03) and motility (r = -0.55, P <.00), and HDS was negatively correlated with sperm density (r= -0.37, P <.00). Conclusions: Sperm chromatin structure assay failed to identify elevated DFI thresholds for negative pregnancy outcome after ART. Patients with low DFI (<= 9%) were least likely to become pregnant, which is also contradictory to SCSA marketing, which states that DFIs of <= 15% have excellent fertility potential. Patients with HDS >= 17% had low pregnancy rates, indicating decreased fertility potential, which deserves further investigation. Larger studies are necessary to confirm that low DFI is asssociated with decreased fertility and , if proved might redefine the use of the SCSA in evaluating infertility.