Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 15, Pages 5282-5286Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00330-10
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Funding
- Australian Research Council
- National Science Foundation [DEB0516347]
- Fulbright Postgraduate Fellowship
- Direct For Biological Sciences
- Division Of Environmental Biology [0964997] Funding Source: National Science Foundation
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A real-time quantitative PCR-based detection assay targeting the dnaJ gene ( encoding heat shock protein 40) of the coral pathogen Vibrio coralliilyticus was developed. The assay is sensitive, detecting as little as 1 CFU per ml in seawater and 104 CFU per cm(2) of coral tissue. Moreover, inhibition by DNA and cells derived from bacteria other than V. coralliilyticus was minimal. This assay represents a novel approach to coral disease diagnosis that will advance the field of coral disease research.
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