Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 77, Issue 5, Pages 1881-1884Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02109-10
Keywords
-
Categories
Funding
- Ministry of Education, Youth, and Sports of the Czech Republic [LC06010, MSM0021622412]
- European Regional Development Fund [CZ.1.05/2.1.00/01.0001]
- Czech Academy of Sciences [IAA401630901]
- Ministry of Education, Culture, Sports, Science, and Technology
- Ministry of Agriculture, Forestry, and Fisheries, Japan
- Grants-in-Aid for Scientific Research [22380047] Funding Source: KAKEN
Ask authors/readers for more resources
We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
