Journal
PLOS MEDICINE
Volume 2, Issue 8, Pages 798-807Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pmed.0020249
Keywords
-
Categories
Funding
- NIAID NIH HHS [AI-49334, 1 R21 AI54260, R01 AI049334, R21 AI054260] Funding Source: Medline
Ask authors/readers for more resources
Background Trials in macaque models play an essential role in the evaluation of biomedical interventions that aim to prevent HIV infection, such as vaccines, microbicides, and systemic chemoprophylaxis. These trials are usually conducted with very high virus challenge doses that result in infection with certainty. However, these high challenge doses do not realistically reflect the low probability of HIV transmission in humans, and thus may rule out preventive interventions that could protect against real life exposures. The belief that experiments involving realistically low challenge doses require large numbers of animals has so far prevented the development of alternatives to using high challenge doses. Methods and Findings Using statistical power analysis, we investigate how many animals would be needed to conduct preclinical trials using low virus challenge doses. We show that experimental designs in which animals are repeatedly challenged with low doses do not require unfeasibly large numbers of animals to assess vaccine or microbicide success. Conclusion Preclinical trials using repeated low-dose challenges represent a promising alternative approach to identify potential preventive interventions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available